Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded human rectal tissue; Antigen retrieval by citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Antibody incubation with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:300), overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded rat pancreas tissue; Antigen retrieval by citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Antibody incubation with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:200), overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Western blotting analysis using anti-Adiponectin Receptor 2 antibody (Cat#52739). Total lysates (40 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
ADIPOR2; Adiponectin Receptor 2; PAQR2; ACDCR2; Progestin And AdipoQ Receptor Family Member II; Progestin And AdipoQ Receptor Family Member 2; Adiponectin Receptor Protein 2
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Adiponectin receptor 2
Isotype
Rabbit IgG
Storage Buffer
Supplied in 0.01M TBS (pH7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry (IHC): 1:100-1:500 Immunocytochemistry (IC): 1:100-1:500
Note
This product is for research use only.
Data
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded human rectal tissue; Antigen retrieval by citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Antibody incubation with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:300), overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded rat pancreas tissue; Antigen retrieval by citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Antibody incubation with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:200), overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Western blotting analysis using anti-Adiponectin Receptor 2 antibody (Cat#52739). Total lysates (40 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded human rectal tissue; Antigen retrieval by citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Antibody incubation with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:300), overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded rat pancreas tissue; Antigen retrieval by citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Antibody incubation with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:200), overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Western blotting analysis using anti-Adiponectin Receptor 2 antibody (Cat#52739). Total lysates (40 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Adiponectin Receptor 2 antibody (Cat#52739, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
