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KD-Validated Anti-Smad2 Recombinant Rabbit Monoclonal Antibody #69512

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Flow cytometric analysis of Smad2 in HAP-1 cells using Smad2 antibody (Cat#69512, 1:2,000). Green, isotype control; red, Smad2.
Immunocytochemical staining of HAP-1 cells with Smad2 antibody (Cat#69512, 1:1,000). Nuclei were stained blue with DAPI; Smad2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Smad2 antibody (Cat#69512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad2 antibody (Cat#69512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Smad2 antibody (Cat#69512). Smad2 expression in wild type (WT) and Smad2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Smad2 antibody (Cat#69512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    SMAD2
  • 货号：
    69512
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Data

Information
Product Name KD-Validated Anti-Smad2 Recombinant Rabbit Monoclonal Antibody
Aliases JV18-1; MADR2; MADH2; Mothers Against Decapentaplegic Homolog 2; MAD Homolog 2; HMAD-2; HSMAD2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2; Sma- And Mad-Related Protein 2; Mothers Against DPP Homolog 2; Mother Against DPP Homolog 2; Mad-Related Protein 2; SMAD 2; CHTD8; Smad2; JV18; LDS6
Background

Gene Name: SMAD2

NCBI Gene Entry: 4087

UniProt Entry: Q15796
Application Information

Molecular Weight: Predicted, 52 kDa, observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB970

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human smad2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Flow cytometric analysis of Smad2 in HAP-1 cells using Smad2 antibody (Cat#69512, 1:2,000). Green, isotype control; red, Smad2.
  • Immunocytochemical staining of HAP-1 cells with Smad2 antibody (Cat#69512, 1:1,000). Nuclei were stained blue with DAPI; Smad2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Smad2 antibody (Cat#69512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad2 antibody (Cat#69512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Smad2 antibody (Cat#69512). Smad2 expression in wild type (WT) and Smad2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Smad2 antibody (Cat#69512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    SMAD2
  • 货号：
    69512
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Smad2 in HAP-1 cells using Smad2 antibody (Cat#69512, 1:2,000). Green, isotype control; red, Smad2.
Immunocytochemical staining of HAP-1 cells with Smad2 antibody (Cat#69512, 1:1,000). Nuclei were stained blue with DAPI; Smad2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Smad2 antibody (Cat#69512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad2 antibody (Cat#69512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Smad2 antibody (Cat#69512). Smad2 expression in wild type (WT) and Smad2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Smad2 antibody (Cat#69512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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