Western blotting analysis of lysates from U2OS cells, primary antibody was diluted at 1:1,000, 4℃ over night.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. Antibody was diluted at 1:200 (4℃ overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 45min).
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
A synthesized peptide derived from human Phospho-MOB1 (T12)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB):1:500-1:2,000 Immunohistochemistry (IHC): 1:50-1:300
Note
This product is for research use only.
Data
Western blotting analysis of lysates from U2OS cells, primary antibody was diluted at 1:1,000, 4℃ over night.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. Antibody was diluted at 1:200 (4℃ overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 45min).
Western blotting analysis of lysates from U2OS cells, primary antibody was diluted at 1:1,000, 4℃ over night.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. Antibody was diluted at 1:200 (4℃ overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200 (room temperature, 45min).
