Western blotting analysis using anti-Phospho-Cdk1/Cdc2 (Thr161) antibody (Cat#U2264). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Cdk1/Cdc2 (Thr161) antibody (Cat#U2264, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
CDK1; Cyclin Dependent Kinase 1; CDC28A; CDC2; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; Cyclin-Dependent Kinase 1; P34 Protein Kinase; P34CDC2; Cell Cycle Controller CDC2; EC 2.7.11.22; EC 2.7.11.23; CDKN1
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Phospho-Cdk1/Cdc2 (Thr161)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry (IHC): 1:100-1:300 Immunocytochemistry (IC): 1:50-1:200
Note
This product is for research use only.
Data
Western blotting analysis using anti-Phospho-Cdk1/Cdc2 (Thr161) antibody (Cat#U2264). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Cdk1/Cdc2 (Thr161) antibody (Cat#U2264, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Cdk1/Cdc2 (Thr161) antibody (Cat#U2264). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Cdk1/Cdc2 (Thr161) antibody (Cat#U2264, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
