Western blotting analysis using anti-Phospho-PI3 Kinase Class III (S249) antibody (Cat#U1944). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PI3 Kinase Class III (S249) antibody (Cat#U1944, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Anti-Phospho-PI3 Kinase Class III (S249) Rabbit Polyclonal Antibody
Aliases
Phosphatidylinositol 3-kinase catalytic subunit type 3 (PI3-kinase type 3) (PI3K type 3) (PtdIns-3-kinase type 3) (EC 2.7.1.137) (Phosphatidylinositol 3-kinase p100 subunit) (Phosphoinositide-3-kinase class 3) (hVps34)
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
A synthesized peptide derived from human Phospho-PI3 Kinase Class III (Ser249)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry-Paraffin (IHC-P): 1:50-1:300
Note
This product is for research use only.
Data
Western blotting analysis using anti-Phospho-PI3 Kinase Class III (S249) antibody (Cat#U1944). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PI3 Kinase Class III (S249) antibody (Cat#U1944, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-PI3 Kinase Class III (S249) antibody (Cat#U1944). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PI3 Kinase Class III (S249) antibody (Cat#U1944, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
