Western blotting analysis using anti-Phospho-c-Fos (S362) antibody (Cat#U1428). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-c-Fos (S362) antibody (Cat#U1428, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using Fos (Phospho-Ser362) Antibody. The picture on the right is blocked with the phospho peptide.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Phospho-c-Fos (Ser362)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry (IHC): 1:100-1:300 Immunocytochemistry (IC): 1:50-1:200
Note
This product is for research use only.
Data
Western blotting analysis using anti-Phospho-c-Fos (S362) antibody (Cat#U1428). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-c-Fos (S362) antibody (Cat#U1428, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using Fos (Phospho-Ser362) Antibody. The picture on the right is blocked with the phospho peptide.
Western blotting analysis using anti-Phospho-c-Fos (S362) antibody (Cat#U1428). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-c-Fos (S362) antibody (Cat#U1428, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using Fos (Phospho-Ser362) Antibody. The picture on the right is blocked with the phospho peptide.
