Western blotting analysis using anti-Phospho-NFκB-p65 (Thr254) antibody (Cat#U1096). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-NFκB-p65 (Thr254) antibody (Cat#U1096, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr254) Antibody. The picture on the right is blocked with the phospho peptide.
A synthesized peptide derived from human Phospho-NFκB-p65 (Thr254)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry (IHC): 1:100-1:300 Immunocytochemistry (IC) 1:50-1:200 Immunoprecipitation (IP): 2-5 ug
Note
This product is for research use only.
Data
Western blotting analysis using anti-Phospho-NFκB-p65 (Thr254) antibody (Cat#U1096). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-NFκB-p65 (Thr254) antibody (Cat#U1096, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr254) Antibody. The picture on the right is blocked with the phospho peptide.
Western blotting analysis using anti-Phospho-NFκB-p65 (Thr254) antibody (Cat#U1096). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-NFκB-p65 (Thr254) antibody (Cat#U1096, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr254) Antibody. The picture on the right is blocked with the phospho peptide.
