Western blotting analysis using anti-phospho-p38 (T180/Y182) antibody (Cat#U0723). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p38 (T180/Y182) antibody (Cat#U0723, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Phospho-p38 (T180/Y182)
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry (IHC): 1:100-1:500 Immunocytochemistry (IC): 1:100-1:500
Note
This product is for research use only.
Data
Western blotting analysis using anti-phospho-p38 (T180/Y182) antibody (Cat#U0723). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p38 (T180/Y182) antibody (Cat#U0723, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-p38 (T180/Y182) antibody (Cat#U0723). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-p38 (T180/Y182) antibody (Cat#U0723, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
