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Human AKR1C2 Knockdown Cell Line (WB-Validated) #C67575

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RT-qPCR analysis. HeLa cells were infected with AKR1C2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. AKR1C2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AKR1C2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

AKR1C2
货号：

C67575
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Information

Data

Information

Product Name	Human AKR1C2 Knockdown Cell Line (WB-Validated)
Aliases	AKR1C2; Aldo-Keto Reductase Family 1 Member C2; DD2; HAKRD; MCDR2; BABP; DDH2; DD; Dihydrodiol Dehydrogenase 2; Bile Acid Binding Protein; 3-Alpha Hydroxysteroid Dehydrogenase, Type III; Testicular 17,20-Desmolase Deficiency; Chlordecone Reductase Homolog HAKRD; 3-Alpha-HSD3; DD/BABP; DD-2; TDD; Aldo-Keto Reductase Family 1, Member C2 (Dihydrodiol Dehydrogenase 2; Bile Acid Binding Protein; 3-Alpha Hydroxysteroid Dehydrogenase, Type III) ; Dihydrodiol Dehydrogenase/Bile Acid-Binding Protein; Trans-1,2-Dihydrobenzene-1,2-Diol Dehydrogenase; Type III 3-Alpha-Hydroxysteroid Dehydrogenase; Type II Dihydrodiol Dehydrogenase; Pseudo-Chlordecone Reductase; Dihydrodiol Dehydrogenase 2; AKR1C-Pseudo; EC 1.1.1.112; EC 1.1.1.209; EC 1.1.1.357; EC 1.1.1.53; EC 1.1.1.62; EC 1.3.1.20; EC 1.-.-.-; EC 1.1.1; SRXY8; HBAB
Background	Gene Name: AKR1C2 NCBI Gene Entry: 1646
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human AKR1C2 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Data

RT-qPCR analysis. HeLa cells were infected with AKR1C2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. AKR1C2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against AKR1C2 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

AKR1C2
货号：

C67575
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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