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Human FARSB Knockdown Cell Line (WB-Validated) #C63865

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RT-qPCR analysis. HeLa cells were infected with FARSB-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. FARSB protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FARSB and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    FARSB
  • 货号：
    C63865
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Data

Information
Product Name Human FARSB Knockdown Cell Line (WB-Validated)
Aliases FARSB; Phenylalanyl-TRNA Synthetase Subunit Beta; FRSB; FARSLB; PheHB; Phenylalanyl-TRNA Synthetase-Like, Beta Subunit; Phenylalanine TRNA Ligase 1, Beta, Cytoplasmic; Phenylalanyl-TRNA Synthetase Beta Subunit; Phenylalanine--TRNA Ligase Beta Subunit; EC 6.1.1.20; PheRS; Phenylalanine-TRNA Synthetase-Like, Beta Subunit; Phenylalanyl-TRNA Synthetase Beta Chain; Phenylalanine-TRNA Ligase Beta Chain; EC 6.1.1; HSPC173; NEDBLLA; RILDBC1; RILDBC; RJBS
Background

Gene Name: FARSB

NCBI Gene Entry: 10056
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human FARSB Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Data
  • RT-qPCR analysis. HeLa cells were infected with FARSB-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. FARSB protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FARSB and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    FARSB
  • 货号：
    C63865
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with FARSB-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. FARSB protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against FARSB and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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