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Human PPID Knockdown Cell Line (WB-Validated) #C62594

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RT-qPCR analysis. HeLa cells were infected with PPID-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PPID protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PPID and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PPID
  • 货号：
    C62594
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Data

Information
Product Name Human PPID Knockdown Cell Line (WB-Validated)
Aliases Peptidylprolyl Isomerase D; CYP-40; CypD; Peptidyl-Prolyl Cis-Trans Isomerase D; Cyclophilin-Related Protein; Cyclophilin 40; Rotamase D; EC 5.2.1.8; PPIase D; 40 KDa Peptidyl-Prolyl Cis-Trans Isomerase D; Peptidylprolyl Isomerase D (Cyclophilin D); 40 KDa Peptidyl-Prolyl Cis-Trans Isomerase; Testicular Tissue Protein Li 147; Cyclophilin-40; Cyclophilin D; CYP40; CYPD
Background

Gene Name: PPID

NCBI Gene Entry: 5481
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human PPID Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Data
  • RT-qPCR analysis. HeLa cells were infected with PPID-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. PPID protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PPID and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    PPID
  • 货号：
    C62594
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with PPID-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. PPID protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against PPID and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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