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KO-Validated Anti-Cyclin Dependent Kinase 6 Recombinant Rabbit Monoclonal Antibody #71144

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Flow cytometric analysis of Cyclin dependent kinase 6 expression in HAP-1 cells using anti-Cyclin dependent kinase 6 antibody (Cat# 71144, 1:2,000). Green, isotype control; red, Cyclin dependent kinase 6.
Western blotting analysis using anti-Cyclin dependent kinase 6 antibody (Cat#71144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin dependent kinase 6 antibody (Cat#71144, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cyclin dependent kinase 6 antibody (Cat#71144). Cyclin dependent kinase 6 expression in wild-type (WT) and cyclin dependent kinase 6 (CDK6) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 6 antibody (Cat#71144, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    CDK6
  • 货号:
    71144
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KO-Validated Anti-Cyclin Dependent Kinase 6 Recombinant Rabbit Monoclonal Antibody
Aliases CDK6; Cyclin Dependent Kinase 6; PLSTIRE; Serine/Threonine-Protein Kinase PLSTIRE; Cell Division Protein Kinase 6; Cyclin-Dependent Kinase 6; EC 2.7.11.22; EC 2.7.11; MCPH12; CDKN6
Background

Gene Name: CDK6

NCBI Gene Entry: 1021

UniProt Entry: Q00534

Application Information

Molecular Weight: Predicted, 37 kDa, observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB540

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CDK6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Data
  • Flow cytometric analysis of Cyclin dependent kinase 6 expression in HAP-1 cells using anti-Cyclin dependent kinase 6 antibody (Cat# 71144, 1:2,000). Green, isotype control; red, Cyclin dependent kinase 6.
  • Western blotting analysis using anti-Cyclin dependent kinase 6 antibody (Cat#71144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin dependent kinase 6 antibody (Cat#71144, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-cyclin dependent kinase 6 antibody (Cat#71144). Cyclin dependent kinase 6 expression in wild-type (WT) and cyclin dependent kinase 6 (CDK6) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 6 antibody (Cat#71144, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Cyclin dependent kinase 6 expression in HAP-1 cells using anti-Cyclin dependent kinase 6 antibody (Cat# 71144, 1:2,000). Green, isotype control; red, Cyclin dependent kinase 6.
Western blotting analysis using anti-Cyclin dependent kinase 6 antibody (Cat#71144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin dependent kinase 6 antibody (Cat#71144, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cyclin dependent kinase 6 antibody (Cat#71144). Cyclin dependent kinase 6 expression in wild-type (WT) and cyclin dependent kinase 6 (CDK6) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 6 antibody (Cat#71144, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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