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KD-Validated Anti-PYCR1 Mouse Monoclonal Antibody #65934

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Flow cytometric analysis of PYCR1 expression in HepG2 cells using anti-PYCR1 antibody (Cat#65934, 1:2,000). Green, isotype control; red, PYCR1.
Western blotting analysis using anti-PYCR1 antibody (Cat#65934). PYCR1 expression in wild-type (WT) and PYCR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PYCR1 antibody (Cat#65934, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PYCR1 antibody (Cat#65934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PYCR1 antibody (Cat#65934, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of PYCR1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PYCR1 antibody (Cat#65934, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-PYCR1 antibody (Cat#65934, 1:1,000), Top panel: wild-type (WT); Bottom panal: PYCR1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PYCR1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    PYCR1
  • 货号：
    65934
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Data

Information
Product Name KD-Validated Anti-PYCR1 Mouse Monoclonal Antibody
Aliases PYCR1; Pyrroline-5-Carboxylate Reductase 1; P5C; Pyrroline-5-Carboxylate Reductase 1, Mitochondrial; P5C Reductase 1; EC 1.5.1.2; Proliferation-Inducing Protein 45; ARCL2B; ARCL3B; P5CR 1; PIG45; PP222; P5CR; PRO3; PYCR
Background

NCBI Gene Entry: 5831

UniProt Entry: P32322
Application Information

Molecular Weight: Predicted, 33 kDa; observed, 31 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5905

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen Recombinant protein of human PYCR1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Flow cytometric analysis of PYCR1 expression in HepG2 cells using anti-PYCR1 antibody (Cat#65934, 1:2,000). Green, isotype control; red, PYCR1.
  • Western blotting analysis using anti-PYCR1 antibody (Cat#65934). PYCR1 expression in wild-type (WT) and PYCR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PYCR1 antibody (Cat#65934, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-PYCR1 antibody (Cat#65934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PYCR1 antibody (Cat#65934, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of PYCR1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PYCR1 antibody (Cat#65934, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-PYCR1 antibody (Cat#65934, 1:1,000), Top panel: wild-type (WT); Bottom panal: PYCR1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PYCR1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    PYCR1
  • 货号：
    65934
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of PYCR1 expression in HepG2 cells using anti-PYCR1 antibody (Cat#65934, 1:2,000). Green, isotype control; red, PYCR1.
Western blotting analysis using anti-PYCR1 antibody (Cat#65934). PYCR1 expression in wild-type (WT) and PYCR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PYCR1 antibody (Cat#65934, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PYCR1 antibody (Cat#65934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PYCR1 antibody (Cat#65934, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of PYCR1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-PYCR1 antibody (Cat#65934, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-PYCR1 antibody (Cat#65934, 1:1,000), Top panel: wild-type (WT); Bottom panal: PYCR1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PYCR1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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