Flow cytometric analysis of MAD2L1BP expression in HAP-1 cells using anti-MAD2L1BP antibody (Cat#65917, 1:2,000). Green, isotype control; red, MAD2L1BP.
Western blotting analysis using anti-MAD2L1BP antibody (Cat#65917). MAD2L1BP expression in wild-type (WT) and MAD2L1BP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-MAD2L1BP antibody (Cat#65917). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAD2L1BP antibody (Cat#65917, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).