KD-Validated Anti-MRI1 Mouse Monoclonal Antibody - 产品中心

KD-Validated Anti-MRI1 Mouse Monoclonal Antibody #65829

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Validation of MRI1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with anti-MRI1 antibody (Cat#65829, 1:2,000) and analyzed using BD flow cytometer.

Western blotting analysis using anti-MRI1 antibody (Cat#65829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MRI1 antibody (Cat#65829, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-MRI1 antibody (Cat#65829). MRI1 expression in wild-type (WT) and MRI1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MRI1 antibody (Cat#65829, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of MRI1 expression in HAP-1 cells using anti-MRI1 antibody (Cat#65829, 1:2,000). Green, isotype control; red, MRI1.

基因名：

MRI1
货号：

65829
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Data

Information

Product Name	KD-Validated Anti-MRI1 Mouse Monoclonal Antibody
Aliases	MRI1; Methylthioribose-1-Phosphate Isomerase 1; MRDI; Mediator Of RhoA-Dependent Invasion; Ypr118w; MtnA; Translation Initiation Factor EIF-2B Subunit Alpha/Beta/Delta-Like Protein; S-Methyl-5-Thioribose-1-Phosphate Isomerase 1; Methylthioribose-1-Phosphate Isomerase; MTR-1-P Isomerase; EC 5.3.1.23; MGC3207; M1Pi; Methylthioribose-1-Phosphate Isomerase Homolog (S. Cerevisiae); Methylthioribose-1-Phosphate Isomerase Homolog; S-Methyl-5-Thioribose-1-Phosphate Isomerase
Background	Gene Name: MRI1 NCBI Gene Entry: 84245 UniProt Entry: Q9BV20
Application Information	Molecular Weight: Predicted, 39 kDa, observed, 41 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB5040 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human MRI1
Isotype	Mouse IgG2b
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Validation of MRI1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with anti-MRI1 antibody (Cat#65829, 1:2,000) and analyzed using BD flow cytometer.
Western blotting analysis using anti-MRI1 antibody (Cat#65829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MRI1 antibody (Cat#65829, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-MRI1 antibody (Cat#65829). MRI1 expression in wild-type (WT) and MRI1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MRI1 antibody (Cat#65829, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MRI1 expression in HAP-1 cells using anti-MRI1 antibody (Cat#65829, 1:2,000). Green, isotype control; red, MRI1.

基因名：

MRI1
货号：

65829
应用：

WB, FCM
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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