KD-Validated Anti-GCLM Recombinant Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-GCLM Recombinant Rabbit Monoclonal Antibody #62007

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Flow cytometric analysis of GCLM expression in HepG2 cells using GCLM antibody (Cat#62007, 1:2,000). Green, isotype control; red, GCLM.

Immunocytochemical staining of HepG2 cells with anti-GCLM antibody (Cat#62007, 1:1,000). Nuclei were stained blue with DAPI; GCLM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-GCLM antibody (Cat#62007). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GCLM antibody (Cat#62007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-GCLM antibody (Cat#62007). GCLM expression in wild type (WT) and GCLM shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-GCLM antibody (Cat#62007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Validation of GCLM knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GCLM antibody (Cat#62007, 1:2,000) and analyzed using BD flow cytometer.

基因名：

GCLM
货号：

62007
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Data

Information

Product Name	KD-Validated Anti-GCLM Recombinant Rabbit Monoclonal Antibody
Aliases	GCLM; Glutamate-Cysteine Ligase Modifier Subunit; GLCLR; Gamma-Glutamylcysteine Synthetase Regulatory Subunit; Glutamate--Cysteine Ligase Regulatory Subunit; Gamma-ECS Regulatory Subunit; GCS Light Chain; Glutamate-Cysteine Ligase (Gamma-Glutamylcysteine Synthetase), Regulatory (30.8kD); Glutamate-Cysteine Ligase Modifier Subunit Delta2 Alternative Splicing; Glutamate-Cysteine Ligase Regulatory Protein; Glutamate-Cysteine Ligase, Modifier Subunit; Glutamate--Cysteine Ligase Modifier Subunit; Gamma-Glutamylcysteine Synthetase; GSC Light Chain
Background	Gene Name: GCLM NCBI Gene Entry: 2730 UniProt Entry: P48507
Application Information	Molecular Weight: Predicted, 31 kDa, observed, 28 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5535 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human GCLM
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of GCLM expression in HepG2 cells using GCLM antibody (Cat#62007, 1:2,000). Green, isotype control; red, GCLM.
Immunocytochemical staining of HepG2 cells with anti-GCLM antibody (Cat#62007, 1:1,000). Nuclei were stained blue with DAPI; GCLM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-GCLM antibody (Cat#62007). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GCLM antibody (Cat#62007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-GCLM antibody (Cat#62007). GCLM expression in wild type (WT) and GCLM shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-GCLM antibody (Cat#62007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of GCLM knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GCLM antibody (Cat#62007, 1:2,000) and analyzed using BD flow cytometer.

基因名：

GCLM
货号：

62007
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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