Flow cytometric analysis of Diacylglycerol O-acyltransferase 1 expression in HeLa cells using Diacylglycerol O-acyltransferase 1 antibody (Cat#61926, 1:2,000). Green, isotype control; red, Diacylglycerol O-acyltransferase 1.
Immunocytochemical staining of Hela cells with anti-Diacylglycerol O-acyltransferase 1 antibody (Cat #61926, 1:1,000) . Nuclei were stained blue with DAPI; Diacylglycerol O-acyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#61926). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#61926, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#61926). Diacylglycerol O-acyltransferase 1 expression in wild type (WT) and Diacylglycerol O-acyltransferase 1 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Diacylglycerol O-acyltransferase 1 antibody (Cat#61926, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
