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KD-Validated Anti-Bridging integrator 1 Recombinant Rabbit Monoclonal Antibody #61353

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Flow cytometric analysis of Bridging integrator 1 expression in C2C12 cells using Bridging integrator 1 antibody (Cat#61353, 1:2,000). Green, isotype control; red, Bridging integrator 1.
Immunocytochemical staining of C2C12 cells with anti-Bridging integrator 1 antibody (Cat#61353, 1:1,000) . Nuclei were stained blue with DAPI; Bridging integrator 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Bridging integrator 1 expression in wild type (WT) and Bridging integrator 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    BIN1
  • 货号：
    61353
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Data

Information
Product Name KD-Validated Anti-Bridging integrator 1 Recombinant Rabbit Monoclonal Antibody
Aliases BIN1; Bridging Integrator 1; Myc Box-Dependent-Interacting Protein 1; Amphiphysin II; SH3P9; AMPH2; AMPHL; Box-Dependent Myc-Interacting Protein 1; Amphiphysin-Like Protein; Amphiphysin 2; Box Dependant MYC Interacting Protein 1; CNM2
Background

Gene Name: BIN1

NCBI Gene Entry: 274

UniProt Entry: O00499
Application Information

Molecular Weight: Predicted, 65 kDa, observed, 50,60,65kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3010

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human BIN1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Flow cytometric analysis of Bridging integrator 1 expression in C2C12 cells using Bridging integrator 1 antibody (Cat#61353, 1:2,000). Green, isotype control; red, Bridging integrator 1.
  • Immunocytochemical staining of C2C12 cells with anti-Bridging integrator 1 antibody (Cat#61353, 1:1,000) . Nuclei were stained blue with DAPI; Bridging integrator 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Bridging integrator 1 expression in wild type (WT) and Bridging integrator 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    BIN1
  • 货号：
    61353
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Bridging integrator 1 expression in C2C12 cells using Bridging integrator 1 antibody (Cat#61353, 1:2,000). Green, isotype control; red, Bridging integrator 1.
Immunocytochemical staining of C2C12 cells with anti-Bridging integrator 1 antibody (Cat#61353, 1:1,000) . Nuclei were stained blue with DAPI; Bridging integrator 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Bridging integrator 1 antibody (Cat#61353). Bridging integrator 1 expression in wild type (WT) and Bridging integrator 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Bridging integrator 1 antibody (Cat#61353, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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