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Anti-HARS1 Mouse Monoclonal Antibody #5359

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Flow cytometric analysis of HARS1 expression in HepG2 cells using anti-HARS1 antibody (Cat#5359, 1:2,000). Green, isotype control; red, HARS1.

Immunocytochemical staining of HepG2 cells with anti-HARS1 antibody (Cat#5359, 1:1,000). Nuclei were stained blue with DAPI; HARS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-HARS1 antibody (Cat#5359). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HARS1 antibody (Cat#5359, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

HARS1
货号：

5359
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-HARS1 Mouse Monoclonal Antibody
Aliases	HARS1; Histidyl-TRNA Synthetase 1; HisRS; HARS; Histidine--TRNA Ligase, Cytoplasmic; Histidyl-TRNA Synthetase; EC 6.1.1.21; USH3B; HRS; Histidine TRNA Ligase 1, Cytoplasmic; Histidine Translase; Usher Syndrome 3B; Jo‐1 Antigen; Jo-1 Antigen; CMT2W
Background	Gene Name: HARS1 NCBI Gene Entry: 3035 UniProt Entry: P12081
Application Information	Molecular Weight: Predicted, 57 kDa; observed, 50 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB1135 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human HARS
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of HARS1 expression in HepG2 cells using anti-HARS1 antibody (Cat#5359, 1:2,000). Green, isotype control; red, HARS1.
Immunocytochemical staining of HepG2 cells with anti-HARS1 antibody (Cat#5359, 1:1,000). Nuclei were stained blue with DAPI; HARS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-HARS1 antibody (Cat#5359). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HARS1 antibody (Cat#5359, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

HARS1
货号：

5359
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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