Western blotting analysis using anti-ISG15 antibody (Cat#52871). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#52871, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human ISG15
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using anti-ISG15 antibody (Cat#52871). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#52871, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ISG15 antibody (Cat#52871). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#52871, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
