Western blotting analysis using anti-SIRT6 antibody (Cat#52865). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-SIRT6 antibody (Cat#52865, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SIRT6; Sirtuin 6; Protein Mono-ADP-Ribosyltransferase Sirtuin-6; NAD-Dependent Protein Deacetylase Sirtuin-6; NAD-Dependent Protein Deacylase Sirtuin-6; Regulatory Protein SIR2 Homolog 6; SIR2-Like Protein 6; SIR2L6; HSIRT6; Sirtuin (Silent Mating Type Information Regulation 2, S. Cerevisiae, Homolog) 6; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 6 (S. Cerevisiae); Sir2-Related Protein Type 6; Sirtuin Type 6; EC 2.3.1.286; EC 2.3.1.-; EC 2.4.2.-
Applications Tested: Western Blotting (WB), Immunocytochemistry (IC), Immunoprecipitation (IP)
Immunogen
A synthesized peptide derived from human SIRT6
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using anti-SIRT6 antibody (Cat#52865). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-SIRT6 antibody (Cat#52865, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SIRT6 antibody (Cat#52865). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-SIRT6 antibody (Cat#52865, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
