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Anti-ZO-1 Rabbit Monoclonal Antibody #52822

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Western blotting analysis using anti-ZO-1 antibody (Cat#52822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZO-1 antibody (Cat#52822, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunofluorescence-tissue was performed on paraffin-embedded mouse brain sections using an anti-ZO-1 antibody (Cat#52822, 1:200). Antigen retrieval was carried out in sodium citrate buffer (pH 6.0). Nuclei were counterstained with DAPI (blue), and ZO-1 was visualized with Alexa Fluor® 647 (magenta). Protein abundance, based on laser intensity and smart gain settings, was classified as medium. Images were acquired using a Leica Stellaris 5 confocal microscope. Scale bar: 20 µm.

基因名：

TJP1
货号：

52822
应用：

WB, IHC, IF
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 900.00，¥ 1,800.00，¥ 3,300.00

Information

Data

Information

Product Name	Anti-ZO-1 Rabbit Monoclonal Antibody
Aliases	TJP1; Tight Junction Protein 1; Tight Junction Protein ZO-1; ZO-1; Zona Occludens 1; DKFZp686M05161; MGC133289; Zonula Occludens 1 Protein; Zonula Occludens Protein 1; Zona Occludens Protein 1; ZO1
Background	Gene Name: TJP1 NCBI Gene Entry: 7082 UniProt Entry: Q07157
Application Information	Molecular Weight: Predicted, 195 kDa; observed, 220 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB7010 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunofluorescence-tissue (IF-Tissue)
Immunogen	Recombinant protein of human ZO-1
Isotype	Rabbit IgG
Storage Buffer	Supplied in 0.01M TBS (pH7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin300.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:2,500 Immunohistochemistry (IHC): 1:100-1:500 Immunofluorescence-Tissue (IF-Tissue): 1:100-1:200
Note	This product is for research use only.

Data

Western blotting analysis using anti-ZO-1 antibody (Cat#52822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZO-1 antibody (Cat#52822, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunofluorescence-tissue was performed on paraffin-embedded mouse brain sections using an anti-ZO-1 antibody (Cat#52822, 1:200). Antigen retrieval was carried out in sodium citrate buffer (pH 6.0). Nuclei were counterstained with DAPI (blue), and ZO-1 was visualized with Alexa Fluor® 647 (magenta). Protein abundance, based on laser intensity and smart gain settings, was classified as medium. Images were acquired using a Leica Stellaris 5 confocal microscope. Scale bar: 20 µm.

基因名：

TJP1
货号：

52822
应用：

WB, IHC, IF
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 900.00，¥ 1,800.00，¥ 3,300.00

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