Western blotting analysis using anti-IFNG antibody (Cat#52522). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IFNG antibody (Cat#52522, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of IFNG antibody in paraffin-embedded human lymph node tissue.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human IFN-gamma
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry (IHC): 1:100-1:300 Immunocytochemistry (IC): 1:50-1:200
Note
This product is for research use only.
Data
Western blotting analysis using anti-IFNG antibody (Cat#52522). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IFNG antibody (Cat#52522, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of IFNG antibody in paraffin-embedded human lymph node tissue.
Western blotting analysis using anti-IFNG antibody (Cat#52522). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IFNG antibody (Cat#52522, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry analysis of IFNG antibody in paraffin-embedded human lymph node tissue.
