Western blotting analysis using anti-IL1B antibody (Cat#52174). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IL1B antibody (Cat#52174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human IL-1β
Isotype
Mouse IgG1 kappa
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.05% Proclin 300 and 0.05%BSA.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunocytochemistry (IC): 1:100-1:500
Note
This product is for research use only.
Data
Western blotting analysis using anti-IL1B antibody (Cat#52174). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IL1B antibody (Cat#52174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-IL1B antibody (Cat#52174). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-IL1B antibody (Cat#52174, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
