Anti-Phospho-PKC alpha (T497) Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-PKC alpha (T497) Recombinant Rabbit Monoclonal Antibody #4625

一键复制产品信息

返回列表

Flow cytometric analysis of Phospho-PKC alpha (T497) expression in C2C12 cells using anti-Phospho-PKC alpha (T497) antibody (Cat#4625, 1:2,000). Green, isotype control; red, Phospho-PKC alpha (T497).

Immunocytochemical staining of C2C12 cells with anti-phospho-PKC alpha (T497) antibody (Cat#4625, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PKC alpha (T497) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-phospho-PKC alpha (T497) antibody (Cat#4625). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PKC alpha (T497) antibody (Cat#4625, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PRKCA
货号：

4625
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-Phospho-PKC alpha (T497) Recombinant Rabbit Monoclonal Antibody
Aliases	PRKCA; Protein Kinase C Alpha; PKCA; Protein Kinase C Alpha Type; EC 2.7.11.13; PKC-Alpha; PRKACA; PKC-A; Protein Kinase C, Alpha; Aging-Associated Gene 6; EC 2.7.11; PKCalpha; PKCI+/-; PKCα; AAG6
Background	Gene Name: PRKCA NCBI Gene Entry: 5578 UniProt Entry: P17252
Application Information	Molecular Weight: Predicted, 77 kDa; observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12135 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-PKC alpha (T497)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of Phospho-PKC alpha (T497) expression in C2C12 cells using anti-Phospho-PKC alpha (T497) antibody (Cat#4625, 1:2,000). Green, isotype control; red, Phospho-PKC alpha (T497).
Immunocytochemical staining of C2C12 cells with anti-phospho-PKC alpha (T497) antibody (Cat#4625, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PKC alpha (T497) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-PKC alpha (T497) antibody (Cat#4625). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PKC alpha (T497) antibody (Cat#4625, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PRKCA
货号：

4625
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页