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Anti-Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody #4470

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Flow cytometric analysis of glucose-6-phosphate isomerase expression in Hela cells using anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:2,000). Green, isotype control; red, glucose-6-phosphate isomerase.
Immunocytochemical staining of Hela cells with anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:1,000). Nuclei were stained blue with DAPI; Glucose-6-phosphate isomerase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-glucose-6-phosphate isomerase antibody (Cat#4470). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    GPI
  • 货号:
    4470
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody
Aliases GPI; Glucose-6-Phosphate Isomerase; AMF; NLK; Autocrine Motility Factor; Phosphoglucose Isomerase; Phosphohexose Isomerase; Neuroleukin; EC 5.3.1.9; SA-36; PGI; PHI; Hexose Monophosphate Isomerase; Glucose Phosphate Isomerase; Hexosephosphate Isomerase; Phosphosaccharomutase; Phosphohexomutase; Sperm Antigen-36; Sperm Antigen 36; Oxoisomerase; GNPI; SA36
Background

Gene Name: GPI

NCBI Gene Entry: 2821

UniProt Entry: P06744

Application Information

Molecular Weight: Predicted, 63 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11380

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Glucose 6 phosphate isomerase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Flow cytometric analysis of glucose-6-phosphate isomerase expression in Hela cells using anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:2,000). Green, isotype control; red, glucose-6-phosphate isomerase.
  • Immunocytochemical staining of Hela cells with anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:1,000). Nuclei were stained blue with DAPI; Glucose-6-phosphate isomerase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-glucose-6-phosphate isomerase antibody (Cat#4470). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of glucose-6-phosphate isomerase expression in Hela cells using anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:2,000). Green, isotype control; red, glucose-6-phosphate isomerase.
Immunocytochemical staining of Hela cells with anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:1,000). Nuclei were stained blue with DAPI; Glucose-6-phosphate isomerase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-glucose-6-phosphate isomerase antibody (Cat#4470). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glucose-6-phosphate isomerase antibody (Cat#4470, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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