Anti-Integrin alpha 5 Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-Integrin alpha 5 Recombinant Rabbit Monoclonal Antibody #4263

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Flow cytometric analysis of Integrin alpha 5 expression in C2C12 cells using anti-Integrin alpha 5 antibody (Cat#4263, 1:2,000). Green, isotype control; red, Integrin alpha 5.

Immunocytochemical staining of C2C12 cells with anti-Integrin alpha 5 antibody (Cat#4263, 1:1,000) . Nuclei were stained blue with DAPI; Integrin alpha 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-integrin alpha 5 antibody (Cat#4263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin alpha 5 antibody (Cat#4263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ITGA5
货号：

4263
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-Integrin alpha 5 Recombinant Rabbit Monoclonal Antibody
Aliases	CD49e; FNRA; Integrin, Alpha 5 (Fibronectin Receptor, Alpha Polypeptide); Fibronectin Receptor, Alpha Polypeptide; Fibronectin Receptor Subunit Alpha; CD49 Antigen-Like Family Member E; Integrin Alpha-5; Integrin Alpha-F; VLA-5; Very Late Activation Protein 5, Alpha Subunit; Fibronectin Receptor, Alpha Subunit; ITGA5/SLC9B1 Fusion; CD49e Antigen; VLA5A
Background	Gene Name: ITGA5 NCBI Gene Entry: 3678 UniProt Entry: P08648
Application Information	Molecular Weight: Predicted, 115 kDa; observed, 150 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10345 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human integrin alpha 5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of Integrin alpha 5 expression in C2C12 cells using anti-Integrin alpha 5 antibody (Cat#4263, 1:2,000). Green, isotype control; red, Integrin alpha 5.
Immunocytochemical staining of C2C12 cells with anti-Integrin alpha 5 antibody (Cat#4263, 1:1,000) . Nuclei were stained blue with DAPI; Integrin alpha 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-integrin alpha 5 antibody (Cat#4263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin alpha 5 antibody (Cat#4263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ITGA5
货号：

4263
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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