Anti-A-kinase anchoring protein 8 Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-A-kinase anchoring protein 8 Recombinant Rabbit Monoclonal Antibody #3996

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Flow cytometric analysis of A-kinase anchoring protein 8 expression in C2C12 cells using anti-A-kinase anchoring protein 8 antibody (Cat#3996, 1:2,000). Green, isotype control; red, A-kinase anchoring protein 8.

Immunocytochemical staining of C2C12 cells A-kinase anchoring protein 8 antibody (Cat#3996, 1:1,000). Nuclei were stained blue with DAPI; A-kinase anchoring protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-A-kinase anchoring protein 8 antibody (Cat#3996). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-A-kinase anchoring protein 8 antibody (Cat#3996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

AKAP8
货号：

3996
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-A-kinase anchoring protein 8 Recombinant Rabbit Monoclonal Antibody
Aliases	AKAP8; A-Kinase Anchoring Protein 8; AKAP95; A Kinase (PRKA) Anchor Protein 8; A-Kinase Anchor Protein, 95kDa; A-Kinase Anchor Protein 8; AKAP 95; AKAP-8; A-Kinase Anchor Protein 95 KDa; DKFZp586B1222; DKFZP586B1222; AKAP-95
Background	Gene Name: AKAP8 NCBI Gene Entry: 10270 UniProt Entry: O43823
Application Information	Molecular Weight: Predicted, 76 kDa; observed, 95 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9070 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from AKAP 95
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of A-kinase anchoring protein 8 expression in C2C12 cells using anti-A-kinase anchoring protein 8 antibody (Cat#3996, 1:2,000). Green, isotype control; red, A-kinase anchoring protein 8.
Immunocytochemical staining of C2C12 cells A-kinase anchoring protein 8 antibody (Cat#3996, 1:1,000). Nuclei were stained blue with DAPI; A-kinase anchoring protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-A-kinase anchoring protein 8 antibody (Cat#3996). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-A-kinase anchoring protein 8 antibody (Cat#3996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

AKAP8
货号：

3996
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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