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Anti-GRIN2A Rabbit Monoclonal Antibody #3686

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Flow cytometric analysis of Grin2A expression in HepG2 cells using anti-Grin2A antibody (Cat#3686, 1:2,000). Green, isotype control; red, Grin2A.
Immunocytochemical staining of HepG2 cells with anti-Grin2A antibody (Cat#3686, 1:1,000) . Nuclei were stained blue with DAPI; Grin2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-grin2A antibody (Cat#3686). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-grin2A antibody (Cat#3686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    GRIN2A
  • 货号:
    3686
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-GRIN2A Rabbit Monoclonal Antibody
Aliases GRIN2A; Glutamate Ionotropic Receptor NMDA Type Subunit 2A; GluN2A; NMDAR2A; NR2A; Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2A; N-Methyl D-Aspartate Receptor Subtype 2A; Glutamate Receptor Ionotropic, NMDA 2A; N-Methyl-D-Aspartate Receptor Channel, Subunit Epsilon-1; Glutamate [NMDA] Receptor Subunit Epsilon-1 N-Methyl-D-Aspartate Receptor Subunit 2A; Glutamate Ionotropic Receptor NMDA 2A; HNR2A; EPND; FESD; LKS
Background

Gene Name: GRIN2A

NCBI Gene Entry: 2903

UniProt Entry: Q12879

Application Information

Molecular Weight: Predicted, 165 kDa; observed, 250 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6905

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of mouse NMDAR2A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Flow cytometric analysis of Grin2A expression in HepG2 cells using anti-Grin2A antibody (Cat#3686, 1:2,000). Green, isotype control; red, Grin2A.
  • Immunocytochemical staining of HepG2 cells with anti-Grin2A antibody (Cat#3686, 1:1,000) . Nuclei were stained blue with DAPI; Grin2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-grin2A antibody (Cat#3686). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-grin2A antibody (Cat#3686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Grin2A expression in HepG2 cells using anti-Grin2A antibody (Cat#3686, 1:2,000). Green, isotype control; red, Grin2A.
Immunocytochemical staining of HepG2 cells with anti-Grin2A antibody (Cat#3686, 1:1,000) . Nuclei were stained blue with DAPI; Grin2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-grin2A antibody (Cat#3686). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-grin2A antibody (Cat#3686, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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