Flow cytometric analysis of NFKB inhibitor alpha expression in C2C12 cells using anti-NFKB inhibitor alpha antibody (Cat#3463, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.
Immunocytochemical staining of C2C12 cells with anti-NFKBI inhibitor alpha antibody (Cat#3463, 1:1,000) . Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#3463). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#3463, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of NFKB inhibitor alpha expression in C2C12 cells using anti-NFKB inhibitor alpha antibody (Cat#3463, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.
Immunocytochemical staining of C2C12 cells with anti-NFKBI inhibitor alpha antibody (Cat#3463, 1:1,000) . Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#3463). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#3463, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Flow cytometric analysis of NFKB inhibitor alpha expression in C2C12 cells using anti-NFKB inhibitor alpha antibody (Cat#3463, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.
Immunocytochemical staining of C2C12 cells with anti-NFKBI inhibitor alpha antibody (Cat#3463, 1:1,000) . Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#3463). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#3463, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
