Anti-BCLAF1 Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-BCLAF1 Recombinant Rabbit Monoclonal Antibody #3037

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Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-BCL2 associated transcription factor 1 antibody (Cat#3037, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Flow cytometric analysis of BCL2 associated transcription factor 1 expression in HepG2 cells using anti-BCL2 associated transcription factor 1 antibody (Cat#3037, 1:2,000). Green, isotype control; red, BCL2 associated transcription factor 1.

Immunocytochemical staining of HepG2 cells with anti-BCL2 associated transcription factor 1 antibody(Cat#3037, 1:1000) . Nuclei were stained blue with DAPI; BCL2 associated transcription factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-BCL2 associated transcription factor 1 antibody (Cat#3037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCL2 associated transcription factor 1 antibody (Cat#3037, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BCLAF1
货号：

3037
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-BCLAF1 Recombinant Rabbit Monoclonal Antibody
Aliases	BCLAF1; BCL2 Associated Transcription Factor 1; BTF; BCLAF1 And THRAP3 Family Member 1; KIAA0164; Bcl-2-Associated Transcription Factor 1; BCL2-Associated Transcription Factor 1; BK211L9.1; Btf
Background	Gene Name: BCLAF1 NCBI Gene Entry: 9774 UniProt Entry: Q9NYF8
Application Information	Molecular Weight: Predicted, 106 kDa, observed, 150 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3750 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), Immunohistochemistry (IHC)
Immunogen	A synthesized peptide derived from human BTF
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000 Immunohistochemistry (IHC): 1:100-1:200
Note	This product is for research use only.

Data

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-BCL2 associated transcription factor 1 antibody (Cat#3037, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of BCL2 associated transcription factor 1 expression in HepG2 cells using anti-BCL2 associated transcription factor 1 antibody (Cat#3037, 1:2,000). Green, isotype control; red, BCL2 associated transcription factor 1.
Immunocytochemical staining of HepG2 cells with anti-BCL2 associated transcription factor 1 antibody(Cat#3037, 1:1000) . Nuclei were stained blue with DAPI; BCL2 associated transcription factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BCL2 associated transcription factor 1 antibody (Cat#3037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCL2 associated transcription factor 1 antibody (Cat#3037, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BCLAF1
货号：

3037
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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