Anti-Dysferlin Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-Dysferlin Recombinant Rabbit Monoclonal Antibody #2933

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Flow cytometric analysis of Dysferlin expression in HT-1080 cells using anti-Dysferlin antibody (Cat#2933, 1:2,000). Green, isotype control; red, Dysferlin.

Immunocytochemical staining of HT-1080 cells with anti-Dysferlin antibody (Cat#2933, 1:1,000) . Nuclei were stained blue with DAPI; Dysferlin was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-Dysferlin antibody (Cat#2933). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Dysferlin antibody (Cat#2933, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

DYSF
货号：

2933
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-Dysferlin Recombinant Rabbit Monoclonal Antibody
Aliases	DYSF; Dysferlin; FER1L1; Limb Girdle Muscular Dystrophy 2B (Autosomal Recessive); Fer-1-Like Family Member 1; Fer-1-Like Protein 1; LGMD2B; Dystrophy-Associated Fer-1-Like Protein; Dystrophy-Associated Fer-1-Like 1; LGMDR2; MMD1
Background	Gene Name: DYSF NCBI Gene Entry: 8291 UniProt Entry: O75923
Application Information	Molecular Weight: Predicted, 237 kDa; observed, 250 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB3235 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Dysferlin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of Dysferlin expression in HT-1080 cells using anti-Dysferlin antibody (Cat#2933, 1:2,000). Green, isotype control; red, Dysferlin.
Immunocytochemical staining of HT-1080 cells with anti-Dysferlin antibody (Cat#2933, 1:1,000) . Nuclei were stained blue with DAPI; Dysferlin was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Dysferlin antibody (Cat#2933). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Dysferlin antibody (Cat#2933, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

DYSF
货号：

2933
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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