Anti-ISL1 Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-ISL1 Recombinant Rabbit Monoclonal Antibody #2195

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Flow cytometric analysis of ISL1 expression in HeLa cells using ISL1 antibody (Cat#2195, 1:2,000). Green, isotype control; red, ISL1.

Immunocytochemical staining of HeLa cells with anti-ISL1 antibody (Cat#2195, 1:1,000). Nuclei were stained blue with DAPI; ISL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-ISL1 antibody (Cat#2195). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISL1 antibody (Cat#2195, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ISL1
货号：

2195
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-ISL1 Recombinant Rabbit Monoclonal Antibody
Aliases	ISL1; ISL LIM Homeobox 1; ISLET1; Insulin Gene Enhancer Protein ISL-1; Islet-1; Isl-1; ISL1 Transcription Factor, LIM/Homeodomain, (Islet-1); ISL-1
Background	Gene Name: ISL1 NCBI Gene Entry: 3670 UniProt Entry: P61371
Application Information	Molecular Weight: Predicted, 39 kDa; observed, 39 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6450 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Islet1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of ISL1 expression in HeLa cells using ISL1 antibody (Cat#2195, 1:2,000). Green, isotype control; red, ISL1.
Immunocytochemical staining of HeLa cells with anti-ISL1 antibody (Cat#2195, 1:1,000). Nuclei were stained blue with DAPI; ISL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ISL1 antibody (Cat#2195). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISL1 antibody (Cat#2195, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ISL1
货号：

2195
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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