Anti-Phospho-EGFR (Y1068) Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-EGFR (Y1068) Recombinant Rabbit Monoclonal Antibody #1642

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Flow cytometric analysis of Phospho-EGFR (Y1068) expression in HepG2 cells using Phospho-EGFR (Y1068) antibody (Cat#1642, 1:2,000). Green, isotype control; red, Phospho-EGFR (Y1068).

Immunocytochemical staining of HepG2 cells with anti-Phospho-EGFR (Y1068) antibody (Cat#1642, 1:1,000). Nuclei were stained blue with DAPI; Phospho-EGFR (Y1068)was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-EGFR (Y1068) antibody (Cat#1642). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-EGFR (Y1068) antibody (Cat#1642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-phospho-EGFR (Y1068) antibody (Cat#1642, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

基因名：

EGFR
货号：

1642
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-Phospho-EGFR (Y1068) Recombinant Rabbit Monoclonal Antibody
Aliases	EGFR; Epidermal Growth Factor Receptor; ERBB1; ERRP; ERBB; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Epidermal Growth Factor Receptor Tyrosine Kinase Domain; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EGFR VIII; EC 2.7.10; NISBD2; PIG61; MENA
Background	Gene Name: EGFR NCBI Gene Entry: 1956 UniProt Entry: P00533
Application Information	Molecular Weight: Predicted, 134 kDa; observed, 170 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3890 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), Immunohistochemistry (IHC)
Immunogen	A synthesized peptide derived from human Phospho-EGFR (Y1068)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000 Immunohistochemistry (IHC): 1:100-1:200
Note	This product is for research use only.

Data

Flow cytometric analysis of Phospho-EGFR (Y1068) expression in HepG2 cells using Phospho-EGFR (Y1068) antibody (Cat#1642, 1:2,000). Green, isotype control; red, Phospho-EGFR (Y1068).
Immunocytochemical staining of HepG2 cells with anti-Phospho-EGFR (Y1068) antibody (Cat#1642, 1:1,000). Nuclei were stained blue with DAPI; Phospho-EGFR (Y1068)was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-EGFR (Y1068) antibody (Cat#1642). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-EGFR (Y1068) antibody (Cat#1642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-phospho-EGFR (Y1068) antibody (Cat#1642, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

基因名：

EGFR
货号：

1642
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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