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Anti-DDX17 Recombinant Rabbit Monoclonal Antibody #1422

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Flow cytometric analysis of DDX17 expression in HeLa cells using DDX17 antibody (Cat#1422, 1:2,000). Green, isotype control; red, DDX17.
Immunocytochemical staining of Hela cells with DDX17 antibody (Cat#1422, 1:1,000). Nuclei were stained blue with DAPI; DDX17 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-DDX17 antibody (Cat#1422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX17 antibody (Cat#1422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    DDX17
  • 货号:
    1422
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-DDX17 Recombinant Rabbit Monoclonal Antibody
Aliases DDX17; DEAD-Box Helicase 17; P72; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 17 (72kD); DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 17; Probable ATP-Dependent RNA Helicase DDX17; DEAD (Asp-Glu-Ala-Asp) Box Helicase 17; RNA-Dependent Helicase P72; DEAD Box Protein P72; DEAD Box Protein P82; DEAD Box Protein 17; EC 3.6.4.13; EC 3.6.1; RH70
Background

Gene Name: DDX17

NCBI Gene Entry: 10521

UniProt Entry: Q92841

Application Information

Molecular Weight: Predicted, 80 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3560

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human DDX17
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Flow cytometric analysis of DDX17 expression in HeLa cells using DDX17 antibody (Cat#1422, 1:2,000). Green, isotype control; red, DDX17.
  • Immunocytochemical staining of Hela cells with DDX17 antibody (Cat#1422, 1:1,000). Nuclei were stained blue with DAPI; DDX17 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-DDX17 antibody (Cat#1422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX17 antibody (Cat#1422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DDX17 expression in HeLa cells using DDX17 antibody (Cat#1422, 1:2,000). Green, isotype control; red, DDX17.
Immunocytochemical staining of Hela cells with DDX17 antibody (Cat#1422, 1:1,000). Nuclei were stained blue with DAPI; DDX17 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-DDX17 antibody (Cat#1422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX17 antibody (Cat#1422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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