Anti-OGT Recombinant Rabbit Monoclonal Antibody - 产品中心

Anti-OGT Recombinant Rabbit Monoclonal Antibody #1325

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Flow cytometric analysis of OGT expression in C2C12 cells using OGT antibody (Cat#1325, 1:2,000). Green, isotype control; red, OGT.

Immunocytochemical staining of C2C12 cells with OGT antibody (Cat#1325, 1:1,000). Nuclei were stained blue with DAPI; OGT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-OGT antibody (Cat#1325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-OGT antibody (Cat#1325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

OGT
货号：

1325
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-OGT Recombinant Rabbit Monoclonal Antibody
Aliases	OGT; O-Linked N-Acetylglucosamine (GlcNAc) Transferase; O-GLCNAC; HRNT1; OGT1; O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:Polypeptide-N-Acetylglucosaminyl Transferase); UDP-N-Acetylglucosamine--Peptide N-Acetylglucosaminyltransferase 110 KDa Subunit; UDP-N-Acetylglucosamine:Polypeptide-N-Acetylglucosaminyl Transferase; O-Linked N-Acetylglucosamine Transferase 110 KDa Subunit; O-GlcNAc Transferase Subunit P110; MGC22921; FLJ23071; Uridinediphospho-N-Acetylglucosamine:Polypeptide Beta-N-Acetylglucosaminyl Transferase; O-GlcNAc Transferase P110 Subunit; EC 2.4.1.255; EC 2.4.1.186; HINCUT-1; EC 2.4.1; XLID106; MRX106
Background	Gene Name: OGT NCBI Gene Entry: 8473 UniProt Entry: O15294
Application Information	Molecular Weight: Predicted, 117 kDa; observed, 110 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2505 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human OGT/O-Linked N-Acetylglucosamine Transferase
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Data

Flow cytometric analysis of OGT expression in C2C12 cells using OGT antibody (Cat#1325, 1:2,000). Green, isotype control; red, OGT.
Immunocytochemical staining of C2C12 cells with OGT antibody (Cat#1325, 1:1,000). Nuclei were stained blue with DAPI; OGT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-OGT antibody (Cat#1325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-OGT antibody (Cat#1325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

OGT
货号：

1325
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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