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Anti-CD19 Recombinant Rabbit Monoclonal Antibody #1253

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Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-CD19 antibody (Cat#1253, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Flow cytometric analysis of CD19 expression in HepG2 cells using CD19 antibody (Cat#1253, 1:2,000). Green, isotype control; red, CD19.

Immunocytochemical staining of HepG2 cells with anti-CD19 antibody (Cat#1253, 1:1,000). Nuclei were stained blue with DAPI; CD19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CD19 antibody (Cat#1253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD19 antibody (Cat#1253, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CD19
货号：

1253
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Data

Information

Product Name	Anti-CD19 Recombinant Rabbit Monoclonal Antibody
Aliases	CD19; CD19 Molecule; B-Lymphocyte Surface Antigen B4; T-Cell Surface Antigen Leu-12; Differentiation Antigen CD19; B-Lymphocyte Antigen CD19; CD19 Antigen; CVID3; B4
Background	Gene Name: CD19 NCBI Gene Entry: 930 UniProt Entry: P15391
Application Information	Molecular Weight: Predicted, 61 kDa; observed, 95 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2375 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM), immunohistochemistry (IHC)
Immunogen	A synthesized peptide derived from human CD19
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000 Immunohistochemistry (IHC): 1:100-1:200
Note	This product is for research use only.

Data

Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-CD19 antibody (Cat#1253, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of CD19 expression in HepG2 cells using CD19 antibody (Cat#1253, 1:2,000). Green, isotype control; red, CD19.
Immunocytochemical staining of HepG2 cells with anti-CD19 antibody (Cat#1253, 1:1,000). Nuclei were stained blue with DAPI; CD19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CD19 antibody (Cat#1253). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD19 antibody (Cat#1253, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CD19
货号：

1253
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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